Emerging Begomovirus ToLCNDV Molecular Detection & Chip Grafting Inoculation Transmission Assay under Natural Conditions in Tomato Plants from District Poonch Azad Kashmir & Pakistan
DOI:
https://doi.org/10.70788/ern.1.1.2024.4Keywords:
Begomovirus, Quarantine Pests, Molecular ConfirmationAbstract
Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite, emerging Begomovirus species, usually coupled with satellite molecules and affects tomato cultivation in entire Pakistan. Present study was conducted to evaluate and analyze tomato germplasm against ToLCNDV using Chip graft inoculation assay (CGIA) as most accurate and reliable technique. Molecular studies were conducted for confirmation. According to PCR results, viral contamination was confirmed as ToLCNDV was recorded from all tested seedling samples. Further mechanical transmission confirmed that seedlings from ToLCNDV-infected seeds act as inoculum. Based on disease symptoms developed, plants were classified as tolerant, moderately tolerant, moderately susceptible, and susceptible. It is pertinent to mention that the magnitude of the virus impact particularly on hosts different from Solanaceae and Cucurbitaceae, and seed transmission and ToLCNDV qualify as a potential candidate of Quarantine Pest. Present work is novel study demonstrating ToLCNDV as a seed-transmissible virus in tomato plants from Rawalakot Azad Kashmir & this research further provides a fictitious starting point for plant pathologists suggesting practical implications regarding management approaches.
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